This project, created by Michael Brooks and Tori Marks, is about the colors and chemicals in leaves. Chromatography is the seperation of a mixture by passing it in solution or suspension or as a vapor (as in gas chromatoghraphy) through a medium in which the chemicals seperate the different substances. Through chromotography, we will be able to see the chemicals present in leaves all year round.

Some helpful links to understand this topic better are:
Leaf Chromatography
Leaf Chemicals
Different Colors in Leaves

Materials needed:
Leaves (different colors of each oak, maple, magnolia, and pine)
5 beakers
Labeling pencil
Alcohol solution
9 filter papers
Hot water bath

Procedure:
1. Collect all materials needed to perform the experiment (leaves, solution, filter paper, beakers)
2. Rip leaves up and drop into appropriate beaker
3. Label the beakers according to type and color of leaf
4. Pour about 15 mL of solution into each beaker
5. Put a filter paper in each beaker
6. Pour the heated water into a container and place each beaker into it (hot water bath)
7. Let sit in hot water bath for 30 minutes
8. Record results
Applying the Scientific Method:

Question: How will chemicals be seperated from each other to show the amounts of each one?

Observation: The alcohol had seemed to dissolve some of the colors from the leaves and they were absorbed by the paper and the amount of each chemical depended on how far they rose up the paper.

Hypothesis: The alcohol's acidic level is probably what made the chemicals come off of the leaves and the heat made the chemicals rise up the paper, but each chemical could only move so far that is why we could see the different colors.

Experiment: First we went outside and gathered an assortment of different colors and types of leaves while water in the classroom was being heated. Then when we came back the water would be hot enough to pour into the hot water bath container. Since alcohol is flammable, we could not heat it directly. We put 15 ml of alcohol in each of the 5 beakers. Then we added some of the leaves to the beakers and put chromotograohy paper in with it. We then waited for the hot water and chemicals to "work their magic".

Conclusion: Leaves have many different colors and chemicals in them year round. When leaves are changing color the color on top is really just decaying and the new layer that was there all along is becoming visible. The chemical that is decaying is glucose (a sugar). The maple leaves had a high amount of glucose. You can tell this because they haven't changed much in color yet. So the highest chemical on the Maple paper is most likely glucose.
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